Ovatoxin-a

Oxidation of palytoxins, ovatoxins or ostreocins using periodic acid generates two nitrogen-containing aldehyde fragments; an amino aldehyde common to these toxins, and an amide aldehyde that may vary depending on toxin type.

Journal of The American Society for Mass Spectrometry, Jan 2008 Putative Palytoxin and Its New Analogue, Ovatoxin-a, in Ostreopsis ovata Collected Along the Ligurian Coasts During the 2006 Toxic Outbreak Author CIMINIELLO, Patrizia 1 ; DELL'AVERSANO, Carmela 1 ; FATTORUSSO, Ernesto 1 ; FORINO, Martino 1 ; TARTAGLIONE, Luciana 1 ; GRILLO, Claudio 2 ; MELCHIORRE, Nunzia 2 Availability of pure ovatoxin-a will open the double prospect of fully evaluating its toxicity and preparing reference standards to be employed in LC/MS quantitative analyses. Elucidation of ovatoxin-a’s complex structure will ultimately herald the understanding of the molecular bases of ovatoxins bioactivity. Ovatoxin‐a is the main toxin produced by Ostreopsis ovata, a benthic dinoflagellate that has bloomed massively across the Mediterranean basin over the past years, inflicting both human and environmental suffering.Ovatoxin‐a has recently been isolated from cultures of O. ovata and structurally identified as an analogue of palytoxin: in comparison with palytoxin, ovatoxin‐a lacks three Un derivado anteriormente desconocido de la palitoxina (ovatoxin-A), que se produce como aerosol marino tropical por el dinoflagelado Ostreopsis ovata causó cientos de personas enfermas en Génova, Italia. En 2005 y 2006 enormes floraciones de estas algas aparecieron en el mar Mediterráneo. CiteSeerX - Document Details (Isaac Councill, Lee Giles, Pradeep Teregowda): Abstract: Dinoflagellates of the genus Ostreopsis are known to cause (often fatal) food poisoning in tropical coastal areas following the accumulation of palytoxin (PLTX) and/or its analogues (PLTX group) in crabs, sea urchins or fish. Ostreopsis spp.

06.04.2021 Ovatoxin-a

Learn more about the change. Preparations of palytoxin (PLTX, derived from Japanese Palythoa tuberculosa) and the congeners 42-OH-PLTX (from Hawaiian P. toxica) and ovatoxin-a (isolated from a Japane Putative palytoxin and ovatoxins were detected, being ovatoxin-a the dominant component. The highest concentration per cell almost coincided with maximum Ostreopsis abundances. Novel Aspect: The improved UHPLC-HRMS method evidences the presence of putative palytoxin and ovatoxins in the Ostreopsis bloom in a NW Mediterranean beach.

Ovatoxin A has two oxygen atoms less than PlTX but it is not known from which part of the molecule the oxygen atoms are missing (Ciminiello et al. 2006 (Ciminiello et al. , 2008 Guerrini et al

Among the OVTXs so far detected by high-resolution liquid chromatography coupled to mass spectrometry (HR- LC-MS), ovatoxin-a (OVTX-a) has emerged as the major toxin produced by O. ovata.[3] It was successfully isolated from O. ovata cultures and structurally identified as a paly- toxin analogue by means of NMR techniques. This study provides the first evaluation of the cytotoxic effects of the recently identified palytoxin (PLTX) analog, ovatoxin-a (OVTX-a), the major toxin produced by Ostreopsis cf. ovata in the Mediterranean Sea. Its increasing detection during Ostreopsis blooms and in seafood highlights the need to characterize its toxic effects and to set up appropriate detection methods.

OVTX-a is about 100 fold less potent than PLTX in reducing HaCaT cells viability (EC 50 = 1.1 × 10 –9 M vs 1.8 × 10 –11 M, MTT test) in agreement with a reduced binding affinity (Kd = 1.2 × 10 –9 vs 2.7 × 10 –11 M, saturation experiments on intact cells).

The tissue histopathology of the different toxin preparations by IP and aerosol administration were similar, albeit with some differences. Mar 15, 2018 · Ovatoxin-a was found at the concentration of 78.8 µg/kg only in the soft tissue extract of T0 sea urchins, while the toxin was not detected in C animals 7 and decreased to undetectable levels Results from 2008 and 2009 showed that there is a real danger of human poisoning, as these demonstrated bioaccumulation of the PLTX group (PLTX and ovatoxin-a) in both filter-feeding bivalve molluscs (mussels) and herbivorous echinoderms (sea urchins). The total content accumulated in urchins reached 450 µg PLTX eq/kg total flesh (summer Palytoxin is a polyol marine coelenterate toxin composed of substituted N-3-hydroxypropyl-trans-3-amidoacrylamides and produced by species of Palythoa and Zoanthus soft corals (collectively called zoantharians), either as a defence mechanism or to assist them in capturing prey. Ovatoxin-a MS2 2648.5 MS2 1315.7 MS2 896.2 MS3 727.4 MS3 507.3 MS3 327.1 MS4 309.1 Figure 1.

ovata blooms on the French Mediterranean coast. Z Amzil, M Sibat, N Chomerat, 15 Mar 2018 Ovatoxin-a was found at the concentration of 78.8 µg/kg only in the soft tissue extract of T0 sea urchins, while the toxin was not detected in C Ovatoxin-a and Palytoxin Accumulation in Seafood in. Relation to Ostreopsis cf. ovata Blooms on the French Mediterranean Coast. Marine. Drugs 10, 477-496.

Sep 21, 2013 · Ovatoxin‐a is the main toxin produced by Ostreopsis ovata, a benthic dinoflagellate that has bloomed massively across the Mediterranean basin over the past years, inflicting both human and environmental suffering. Ovatoxin‐a has recently been isolated from cultures of O. ovata and structurally identified as an analogue of palytoxin: in comparison with palytoxin, ovatoxin‐a lacks three most cases ovatoxin-a was the only toxin detected (83 % of samples), and the remaining 17 % of samples contained it in association with ovatoxin-d/e. Alerted by the repeated occurrence of palytoxin-producing species in the Mediterranean, the European Food Safety Authority (EFSA), in its statement on the areas. At least 8 different PlTX analogues are known: PlTX, ostreocin-D, ovatoxin-A, homopalytoxin, bishomopalytoxin, neopalytoxin, deopalytoxin and 42-hydroxypalytoxin, but only for PlTX and ostreocin-D the chemical structure has been characterised. The occurrence data reported by European The aerosol LD50 values (0.063, 0.045, 0.041, and 0.031 μg/kg for the 50:50 mix, 42-OH PLTX, PLTX, and ovatoxin-a, respectively) confirmed the exquisite potency of PLTX suggested by the literature. The tissue histopathology of the different toxin preparations by IP and aerosol administration were similar, albeit with some differences. Mar 15, 2018 · Ovatoxin-a was found at the concentration of 78.8 µg/kg only in the soft tissue extract of T0 sea urchins, while the toxin was not detected in C animals 7 and decreased to undetectable levels Results from 2008 and 2009 showed that there is a real danger of human poisoning, as these demonstrated bioaccumulation of the PLTX group (PLTX and ovatoxin-a) in both filter-feeding bivalve molluscs (mussels) and herbivorous echinoderms (sea urchins).

Ovatoxin‐a has recently been isolated from cultures of O. ovata and structurally identified as an analogue of palytoxin: in comparison with palytoxin, ovatoxin‐a lacks three hydroxy groups at the 17‐, 44‐ and 64‐positions, but features an extra hydroxy functionality at the 42‐position. Herein we report on the NMR‐based elucidation Fragmentation pattern of palytoxin was used as template to gain detailed structural information on ovatoxin-a, the main toxin produced by Ostreopsis ovata, (observe correct font) a benthic dinoflagellate that currently represents the major harmful algal bloom threat in the Mediterranean area. Sep 21, 2013 · Ovatoxin‐a is the main toxin produced by Ostreopsis ovata, a benthic dinoflagellate that has bloomed massively across the Mediterranean basin over the past years, inflicting both human and environmental suffering. Ovatoxin‐a has recently been isolated from cultures of O. ovata and structurally identified as an analogue of palytoxin: in comparison with palytoxin, ovatoxin‐a lacks three most cases ovatoxin-a was the only toxin detected (83 % of samples), and the remaining 17 % of samples contained it in association with ovatoxin-d/e. Alerted by the repeated occurrence of palytoxin-producing species in the Mediterranean, the European Food Safety Authority (EFSA), in its statement on the areas. At least 8 different PlTX analogues are known: PlTX, ostreocin-D, ovatoxin-A, homopalytoxin, bishomopalytoxin, neopalytoxin, deopalytoxin and 42-hydroxypalytoxin, but only for PlTX and ostreocin-D the chemical structure has been characterised.

ovata cells. (1) Lemée, R., Pesando, D., Durand-Clément, M., Dubreuil, A., Meinesz, A., Guerriero, A. & F. Pietra (1993) Preliminary survey of the toxicity of the green alga Ovatoxin A | C129H223N3O52 | CID 90479618 - structure, chemical names, physical and chemical properties, classification, patents, literature, biological activities OVTX-a is about 100 fold less potent than PLTX in reducing HaCaT cells viability (EC 50 = 1.1 × 10 –9 M vs 1.8 × 10 –11 M, MTT test) in agreement with a reduced binding affinity (Kd = 1.2 × 10 –9 vs 2.7 × 10 –11 M, saturation experiments on intact cells). The ovatoxin was quantified using the PLTX standard. The maximum levels found in terms of total PLTX equivalents (PLTX and ovatoxin-a) were approximately 175 µg and 450 µg eq PLTX/kg of total sea urchin flesh, in Iles de Frioul (August 2008) and Villefranche-sur-Mer (July 2008), respectively.

ovata Blooms on the French Mediterranean Coast . By Zouher Amzil, Manoella Sibat, Nicolas Chomerat, Hubert Grossel, Francoise Marco-Miralles, Rodolphe Lemee, Elisabeth Nezan and Veronique Sechet. Cite . Results from 2008 and 2009 showed that there is a real danger of human poisoning, as these demonstrated bioaccumulation of the PLTX group (PLTX and ovatoxin-a) in both filter-feeding bivalve molluscs (mussels) and herbivorous echinoderms (sea urchins). The total content accumulated in urchins reached 450 µg PLTX eq/kg total flesh (summer 2008). Availability of pure ovatoxin-a will open the double prospect of fully evaluating its toxicity and preparing reference standards to be employed in LC/MS quantitative analyses. Elucidation of ovatoxin-a's complex structure will ultimately herald the understanding of the molecular bases of ovatoxins bioactivity.

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Nov 24, 2008 · Two of the most remarkable properties of palytoxin are the large and complex structure (with different analogs, such as ostreocin‐D or ovatoxin‐a) and the extreme acute animal toxicity. The Na + /K + ‐ATPase has been proposed as receptor for palytoxin.

Elucidation of ovatoxin-a's complex structure will ultimately herald the understanding of the molecular bases of ovatoxins bioactivity. Putative palytoxin and its new analogue, ovatoxin-a, in Ostreopsis ovata collected along the ligurian coasts during the 2006 toxic outbreak Main content area. Characterization of ovatoxin-h, a new ovatoxin analog, and evaluation of chromatographic columns for ovatoxin analysis and purification A Anatoxin-a, also known as Very Fast Death Factor (VFDF), is a secondary, bicyclic amine alkaloid and cyanotoxin with acute neurotoxicity.It was first discovered in the early 1960s in Canada, and was isolated in 1972. The toxin is produced by multiple genera of cyanobacteria and has been reported in North America, South America, Central America, Europe, Africa, Asia, and Oceania. Ovatoxin-a, A Palytoxin Analogue Isolated fromOstreopsiscf.ovataFukuyo: Cytotoxic Activity and ELISA Detection Ovatoxin-a and Palytoxin Accumulation in Seafood in Relation to Ostreopsis cf. ovata Blooms on the French Mediterranean Coast Veronique Sechet, Hubert Grossel, Francoise Marco-Miralles, Rodolphe Lemee, Nicolas Chomerat, Manoella Sibat, Zouher Amzil, Elisabeth Nezan A formerly unknown derivative of palytoxin, ovatoxin-a, produced as a marine aerosol by the tropical dinoflagellate Ostreopsis ovata caused hundreds of people in Genoa, Italy, to fall ill.

Ovatoxin-a and Palytoxin Accumulation in Seafood in Relation to Ostreopsis cf. ovata Blooms on the French Mediterranean Coast . By Zouher Amzil, Manoella Sibat, Nicolas Chomerat, Hubert Grossel, Francoise Marco-Miralles, Rodolphe Lemee, Elisabeth Nezan and Veronique Sechet. Cite .

111–120, 2008. View at: Publisher Site | Google Scholar ostreocin-D or ovatoxin-a) and the extreme acute animal toxicity. The Na+⁄K+-ATPase has been proposed as receptor for palytoxin. The marine toxin is known to act on the Na+ pump and elicit an increase in Na+ per-meability, which leads to depolarization and a secondary Ca2+ inﬂux, interfering with some functions of cells. View This Abstract Online; Effects of copper and butyltin compounds on the growth, photosynthetic activity and toxin production of two HAB dinoflagellates: The planktonic Alexandrium catenella and the benthic Ostreopsis cf.